Beginners Immunohistochemistry

The course will provide a sound knowledge of the basics behind IHC and participants will leave having gained the skills to perform and manipulate IHC on tissue to identify one antigen/cellular component in a single tissue section.

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16th - 17th June 2021
Venue: University of Sheffield, SITraN, 385a Glossop Road, Sheffield

Beginners Course in Practical Immunohistochemistry



9.30      Registration

9.45      Introduction

10.00    What is Immunohistochemistry?

11.00    BREAK

11.15    Section Preparation

12.00    Fluorescent IHC

12.30    LUNCH

13.30    How is IHC used in research?

14.00    Maths in IHC

14.45    Devising an IHC protocol (Including a break)

16.00    Practical Session overview


Day 2 – All day practical session

9.15 – 15:00     Staining of paraffin sections with GFAP antibody with break for lunch.

15.00    BREAK

15.15    Microscopy     

16.00    Feedback questionnaire


Event contact details

Lynne Baxter/Dan Fillingham Telephone: 0114 222 2272 Event website


University of Sheffield, SITraN, 385a Glossop Road, Sheffield S10 2HQ