The course will provide a sound knowledge of the basics behind IHC and participants will leave having gained the skills to perform and manipulate IHC on tissue to identify one antigen/cellular component in a single tissue section.
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Beginners Course in Practical Immunohistochemistry
10.00 What is Immunohistochemistry?
11.15 Section Preparation
12.00 Fluorescent IHC
13.30 How is IHC used in research?
14.00 Maths in IHC
14.45 Devising an IHC protocol (Including a break)
16.00 Practical Session overview
Day 2 – All day practical session
9.15 – 15:00 Staining of paraffin sections with GFAP antibody with break for lunch.
16.00 Feedback questionnaire
Event contact details
Lynne Baxter/Dan Fillingham Email: firstname.lastname@example.orgemail@example.com Telephone: 0114 222 2272 Event website
University of Sheffield, SITraN, 385a Glossop Road, Sheffield S10 2HQ