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EXPLORING ELUENT pH IN METHOD DEVELOPMENT Reading

The chromatographic selectivity ‘space’ for various eluent and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included.

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21st March 2017
Venue: Holiday Inn

Eluent phase pH can be a powerful tool for the
chromatographer. Choosing buffer systems and analysis conditions that span an
extended pH range (pH 2 - 11) can have an effect on the retention behaviour and
provide alternative selectivity for many acidic and basic analytes. Changes in analyte
retention from eluent pH can be helpful for screening systems and method
development platforms where observing the number of components in samples is
important before method optimisation is attempted. The chromatographic
selectivity ‘space’ for various eluent and column permutations will be
described for a range of acidic, basic and neutral analyte mixtures and
practical hints and tips for ensuring repeatable and reproducible
chromatography, when working with an extended pH range, will be included.

Event contact details

Jenny Sumner Telephone: 0118 930 3660 Event website

Location

Holiday Inn M4
J10
Reading RG41 5TS