Human Genome Engineering using CRISPR/Cas9

CRISPR, one of the powerful scientific stories of the decade, has the potential to revolutionise several areas of biology including medicine and agriculture. Notably CRIPSR is emerging as a rapid diagnostic tool. Due to its simplicity and reproducibility, CRISPR genome engineering could be used successfully to accelerate both basic and applied research in diseases. The Genome Engineering lab at the University of Westminster aims to explore the potential of CRISPR Cas9 technology to combat cancer treatment. We are intensively involved in training Doctoral and graduate students and have successfully applied CRISPR technology to generate several cellular models to study DNA replication dyamics in cancers. The course will provide insights in to the basics of CRISPR gene – editing and hands-on training in human cell engineering using the robust CRISPR Cas9 system. We will also introduce the attendees to successful sgRNA design and analysis strategies and the state-of-art methods that could be used to address their own genome engineering needs. This CRISPR beginners course is aimed at graduate students and scientists with a good understanding of cellular and molecular biology and interest in human genome editing. The course is relevant for researchers venturing into genome engineering for the first time.

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28th - 30th April 2020
Venue: School of Life Sciences

Specific skills you will learn include:

  • Knowledge of a simple straight forward work flow to generate CRISPR tools
  • Efficient selection and design of guide RNA (gRNA)
  • Delivery of CRIPSR tool in human cells, transfection
  • Validation of genome editing in engineered cells

Attendees will receive a detailed course guide containing all lecture materials, laboratory protocols and troubleshooting tips.

Timetable as follows:

Human Genome Engineering using CRISPR/Cas9                             

Day 1: Tuesday 28th April 2020

9.00 -   9.45                                         Arrivals and registration


9.45-10.00                                     Welcome and introductions


10.00-11.00     Inaugural lecture Human Genome Engineering in human disease modelling current applications and limitations in future

Dr John J Murphy, Research Director, School of Life Sciences

11.00 -11.30       CRISPR in vitro studies: Working on new cancer driver gene?

Case study: My journey with a new cancer breast driver gene and CRISPR/Cas9 genome engineering technology  Nadeen Solaiman, Genome Engineering Lab, UoW


11.30                                             Tea/Coffee BREAK

12.00 -12.30            AGILENT   technologies TALK - Advances in human Genome Engineering

                                 Confirmed, waiting for title

112.30 -13.00 Stages in CRISPR genome editing an introduction, an overview of genomic engineering technologies the design of CRISPR-Cas9 reagents  Dr Kalpana Surendranath

13.00 -14.00                                                  Lunch BREAK

14.00 - 16.30      Guide RNA / Donor design workshop in computer lab  Introduction to: CHOP CHOP, Benchling, Horizon Discovery, Synthego

Advantages and limitations. Simple guide cloning methods and successful transfection sytems  Dr Kalpana Surendranath


16.30 – 17.00                                           Tea/Coffee BREAK


17.00-19.00     Genome Engineering Laboratory C5.02 Lab session:1

Work with cells to be transfected tomorrow, assessing cell count and viability () followed by plating cells for transfection

                                                            END of DAY1


Human Genome Engineering using CRISPR/Cas9

Day 2: Wednesday 29th April 2020

9.30 - 10.00               CRISPR-Cas9 genome editing and the DNA damage response

                                           Dr Kanagaraj Radhakrishnan (Francis Crick Institute)


10.00 – 10.30               THERMOFISHER TALK-Advances in human Genome Engineering

                                     Confirmed waiting for title

10.30 – 11.00                      BREAK

11.00 - 13.00         Lab session:2

                              Transfection of CRISPR components:

                             Preparation of RNP complexes, Electroporation of RNPs and lipofection of CRISPR mcherry vectors

13.00 - 14.00                 Lunch Break

14.00 - 14.30                 Cas9 enzyme purification and applications

14.30 - 15.00      Advances in human Genome Engineering

                            VectorBuilder: CRISPR Vector and Virus solutions

                            Matthew Wheeler,  Associate Director Vector Builder GmbH

15.00 - 15.30                     Coffee break/ networking

15.30-18.00        Lab session:3

           Setting up RNP transfection

           Verification of trial guide design and plan for your first CRISPR editing experiment

 19.00 -21.00                                             Dinner – venue


Human Genome Engineering using CRISPR/Cas9

Day 3: Thursday 30th April 2020

9.30-12.00              Lab session:4     Examine mcherry positive cells via microscopy
                               Knock-In cell validation – Genomic DNA Preparation  Genomic PCR gel analysis of insertions and deletions

12.00 – 13.00                                   Lunch Break

13.00-  14. 00 Advances in human Genome Engineering  Genome Editing: A New Era in Molecular Biology

                         Marc Davies, Account Manager, New England, Biolabs, UK

14.00 – 14.30                  Purification of Cas9 protein: applications 

14.30-15.30                    Verification of genome editing, bioinformatic tools and workflow

15.30 – 16.00                 Summary of the workshop

                                       Guidance and Feedback

16.00 - 16.30                  Closing remarks and acknowledgements

Event contact details

Dr Kalpana Surendranath Telephone: 020 7911 5000 ext. 64121 Event website


School of Life Sciences University of Westminster
115 New Cavendish Street