Virology – Fundamental Laboratory Techniques & Practices
The course has been designed to provide a strong foundation into the principles and practices of working with viruses at Cdntainment Level 2/BSL2. Good aseptic technique is an essential component of cell culture and virus isolation, these elements and good laboratory practices will also be covered in the course. Over the 4 day course the delegates will cover a broad range of virology techniques and topics, the course also provides an excellent opportunity to become part of a far-reaching network of virologists.
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Each lecture is 30 minutes in length. Practical work will involve, aseptic technique, cell culture, sub-culturing & growing cells, infecting cells with virus, quantifying virus in infected cells, harvesting virus, testing sera for antibodies against a virus, extraction of viral nucleic acid and real time PCR. These will be carried out over several 2hr sessions.
A 30 minute tour of a high throughput electron microscopy will be included in the course as will an hour for delegates to meet the experts on site to discuss their specific needs . The course will include a 1h workshop at the end of day two when delegates can discuss, analyse and compare their results from the practical session on day one with the other group members.
A 30 min test will be used to assess understanding of the material covered over the four days.