Events

A week-long celebration across the UK of pathology

We will be explaining the mechanics of the Schemes, including how the statistics are calculated and how to interpret your reports to assist you in troubleshooting.

We will showcase some of the added value extras that the Schemes provide; we are not just a numbers-only service.

We want to give you the chance to ask questions from us on any topic, but perhaps with particular emphasis on how we can assist you in your day job and how to help you with the demands of ISO15189.

Day 1: Set up and run an Isocratic external standard
calibration.

Day 2: Analyse real samples and quantify the analytes
using the calibration set up on the previous day.

Day 1: Set up and run an Isocratic external standard
calibration.
Day 2: Analyse real samples and quantify the analytes
using the calibration set up on the previous day.

This course gives the delegate a systematic review of the facility design, operational practices and safety management which contribute to working safely in the CL3 environment.

To repair/replace major components of the Agilent HPLC system:
Degasser, Pump, Autosampler, column heater and Variable wavelength detector.
To perform a complete operational qualification (OQ) of a system
To discuss the causes for OQ test failure and corrective actions that can be carried out.

To understand which HPLC column to use and why
; To be confident using both normal and reverse phase columns
; To know when to use a pre- and guard columns
; To understand fluorescence and diode array detection methods
; To be able to set up a gradient method
; To determine the gradient dwell volume for a HPLC system
; To analyse cations by ion chromatography
; To understand different calibration methods and perform internal standard calibration

To be able to establish a starting point for Method Development; To know how to use a gradient run to decide whether to use isocratic or gradient elution; To be able to select the most appropriate mode of HPLC e.g. Reversed phase, Normal phase, Ion exchange e.t.c.; To know the parameters that need to be optimised, the order in which to optimise them and to recognise optimum conditions for each sample; To know when to use internal or external standards; To establish confidence in peak identification

To understand which HPLC column to use and why; To be confident using both normal and reverse phase columns; To know when to use a pre- and guard column; To appreciate fluorescence and diode array detection methods; To be able to set up a gradient method; To determine the gradient dwell volume for a HPLC system; To analyse cations by ion chromatography; To understand different calibration methods and perform internal standard calibration

To identify common maintenance problems for Agilent HPLC systems.
To be able to carry out service procedures for the following Agilent HPLC system components:
HPLC Pumps;
Autosampler;
Column compartment;
UV-Vis Detectors;