Getting the most from your Confocal Course July 2019
This Course utilises many different sample types and fluorescent probes (DNA stains, classic antibody labels and fluorescent proteins) and these are chosen to best demonstrate particular problems and techniques. Focus is on the techniques they enable and the problems they generate, which will be applicable to any sample types.
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Day 1
- The delegate/student will know
- Why, when and how to optimise the pinhole,
- How to achieve optimal resolution,
- How to take multicolour images, the correct controls and different approaches.
- Acquire 3D z-stack images and things to consider
- How to vary signal to noise with laser power and voltage gain
- How to use reflection
- Acquire tiled images
- Differences between lenses
Day 2
- Undertake live cell imaging
- How and why change the speed of image acquisition
- How to take dynamic images
- How to optimise time lapse images
- How to remove bleadthrough from muticolour images
- How to use spectral unmixing
Event contact details
Kate Jermey Email: katejermey@rms.org.uk Telephone: 01865 254776 Event website