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To understand which HPLC column to use and why; To be confident using both normal and reverse phase columns; To know when to use a pre- and guard column; To appreciate fluorescence and diode array detection methods; To be able to set up a gradient method; To determine the gradient dwell volume for a HPLC system; To analyse cations by ion chromatography; To understand different calibration methods and perform internal standard calibration

To be able to establish a starting point for Method Development; To know how to use a gradient run to decide whether to use isocratic or gradient elution; To be able to select the most appropriate mode of HPLC e.g. Reversed phase, Normal phase, Ion exchange e.t.c.; To know the parameters that need to be optimised, the order in which to optimise them and to recognise optimum conditions for each sample; To know when to use internal or external standards; To establish confidence in peak identification

To correctly install a HPLC system, including fluids and electrical components; To understand symptoms and; attribute them to certain abnormalities; To be able to perform routine maintenance; To be able to implement corrective measures once faults are spotted e.g. stopping leaks, replacing flow cells and making fittings; To be able to solve retention time, baseline, tailing peaks and back pressure problems

To understand which HPLC column to use and why; To be confident using both normal and reverse phase columns; To know when to use a pre- and guard column; To appreciate fluorescence and diode array detection methods; To be able to set up a gradient method; To determine the gradient dwell volume for a HPLC system; To analyse cations by ion chromatography; To understand different calibration methods and perform internal standard calibration

To be able to establish a starting point for Method Development; To know how to use a gradient run to decide whether to use isocratic or gradient elution; To be able to select the most appropriate mode of HPLC e.g. Reversed phase, Normal phase, Ion exchange e.t.c.; To know the parameters that need to be optimised, the order in which to optimise them and to recognise optimum conditions for each sample; To know when to use internal or external standards; To establish confidence in peak identification

To correctly install a HPLC system, including fluids and electrical components; To understand symptoms and attribute them to certain abnormalities; To be able to perform routine maintenance; To be able to implement corrective measures once faults are spotted e.g. stopping leaks, replacing flow cells and making fittings; To be able to solve retention time, baseline, tailing peaks and back pressure problems

To understand which HPLC column to use and why
; To be confident using both normal and reverse phase columns
; To know when to use a pre- and guard columns
; To understand fluorescence and diode array detection methods
; To be able to set up a gradient method
; To determine the gradient dwell volume for a HPLC system
; To analyse cations by ion chromatography
; To understand different calibration methods and perform internal standard calibration

At the end of the three weeks attendees will have a good overall understanding of most HPLC techniques.
Attendees will gain hands on experience of most HPLC techniques.
Attendees should be able to troubleshoot HPLC problems.
Attendees will become more confident when interpreting HPLC results.

To understand what is meant by HPLC
To appreciate the history and theoretical background of HPLC
To know the requirements for HPLC sample preparation
To be able to make up a sample and mobile phase
To safely operate an isocratic HPLC system
To perform analysis following a HPLC method
To identify and state the function of each component of an HPLC instrument
To know if a chromatogram is integrated correctly

To understand what is meant by HPLC
To appreciate the history and theoretical background of HPLC
To know the requirements for HPLC sample preparation
To be able to make up a sample and mobile phase
To safely operate an isocratic HPLC system
To perform analysis following a HPLC method
To identify and state the function of each component of an HPLC instrument
To know if a chromatogram is integrated correctly