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In order to detect and identify amyloid, biopsy slides must be properly prepared, stained and examined. This requires precise technique, correct equipment and professional expertise, or amyloid deposits may be missed or incorrectly diagnosed. We will look at amyloid staining, correctly diagnosing amyloid in tissues and the associated laboratory techniques.

In the first part of this webinar we will explore the structure, function, and cells of the marrow, and discuss the clinical importance of the bone marrow trephine biopsy. In the second part of this webinar we will explore fixation, decalcification, staining of the bone marrow trephine biopsy, and discuss the challenges we face.

On completion of this webinar students will have a basic knowledge of Principles of immunofluorescence, Optimal specimen preparation for DIF, Practical aspects of cryotomy and processing, DIF imaging considerations, Interpretation of DIF, Diagnostic implications of DIF findings.

“From sectioning to interpretation”, a simple overview of the basic ICC processes.

Discussing the different types of staining mechanisms utilised with the routine special demonstration technique protocols in clinical laboratories.

During this session we will look more closely at the benefits of Mohs micrographic surgery to include improved preservation of uninvolved skin, better cosmetic outcomes and how it can be used on problematic infiltrative invasive tumour types. In addition we will consider the technical requirements in detail along with an understanding of the artefacts that can be created during the procedures and how they can also be resolved. Emphasis will be given to developing a comprehensive understanding of the skills required for the laboratory scientist to deliver good quality stained tissue sections.

Discussing the clinical importance of renal biopsies, and the associated laboratory techniques and challenges.

Discussing extra cellular proteins fibrin and amyloid along with the application and principles of carbohydrate and mucin classification/demonstration.

An in-depth look at the role of Transmission Electron Microscopy in a modern cellular pathology department. What technical challenges are there in preparing tissue for TEM and what diagnostic challenges does TEM have an important role in.

Introduction to basic tissue recognition and discussing and demonstrating epithelia and basic connective tissue